• Product NameWDFY3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total WDFY3 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic protein corresponding to residues near the C terminal of human WDFY3
• Target NameWDFY3
• Alternative Names    ALFY; ZFYVE25
• Accession No. Swiss-Prot:Q8IZQ1
  NCBI Gene ID:23001
  NCBI Protein:BC015214
• UniprotQ8IZQ1
• Gene ID23001;
• Calculated MW 395 kDa
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1: 50-300

This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy.

• Gel: 6%SDS-PAGE
  lysate: 40 ¦Ìg, Lane: Hela cell lysate,
  Primary antibody: 46709£¨WDFY3 Antibody) at dilution 1/650 dilution,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 1 second
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46709(WDFY3 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 46709(WDFY3 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting