• Product NameSurvivin Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB ICC
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to amino acids near the carboxy terminus of human survivin.
• Target NameSurvivin
• Alternative Names    TIAP
• Accession No. Swiss-Prot:O15392
  Gene ID:332
• UniprotO15392
• Gene ID332;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 17 kd
  

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.

• Western blot analysis of survivin in MOLT4 cell lysate in the absence (A) or presence (B) of blocking peptide with survivin antibody at 1 ug/mL.
• Immunocytochemistry staining of MOLT4 cells using survivin at 10 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting