• Product NameRac1(Phospho-Ser71) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Rac1 only when phosphorylated at serine 71.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 71 (P-L-S(p)-Y-P) derived from Human Rac1.
• Target NameRac1
• ModificationPhospho
• Alternative Names    Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; p21-Rac1
• Accession No. Swiss-Prot: P63000
  NCBI Protein: NP_008839.2
• UniprotP63000
• Gene ID5879;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 28kd
  Western blotting: 1:500~1:1000
  

Rac1 encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Aoki K, et al. (2005) Mol Biol Cell; 16(5): 2207

• Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with PMA(lane 2) using Rac1(Phospho-Ser71) Antibody #11203.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting