• Product NameHuman IL-6 ELISA kit
• Brief DescriptionELISA Kit
• ApplicationsELISA
• Species ReactivityHu
• SpecificityNatural and recombinant Human IL-6 Ligand
• Crossing ReactivityNo significant interference observed with available related molecules.
• Target NameHuman IL-6

• Detect Range: 3.9-250pg/ml
  Sensitivity: 2pg/mL
  Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
  Sample Volume: 20 uL
  Assay Time: 3 hours
  Detection method: Colorimetric

• • Aluminium pouches with a Microwell Plate coated with antibody to human IL-6 (8 x 12)
  • 1 vials human IL-6 Standard lyophilized, 1000 pg/ml upon reconstitution
  • 1 vials concentrated Biotin-Conjugate anti-human IL-6 antibody
  • 1 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 2 pieces Adhesive Films
  • package insert

Interleukin 6(IL-6) is a multifunctional protein produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. For reviews on IL-6, see references 1-5.

The human IL-6 cDNA sequence predicts a protein of 212 amino acid (aa) residues in length with two potential N-glycosylation sites. The hydrophobic N-terminal 28 aa residue signal peptide is cleaved to produce a mature protein of 184 amino acids with four cysteine residues and a predicted molecular mass of 21 kDa (6-9). The mouse IL-6 cDNA sequence shows a homology of 42% at the aa level when compared with the human sequence (10). On the basis of sequence similarity and gene structural motif similarity, IL-6 can be grouped in a family of cytokines that also includes OSM, G-CSF, LIF, and CNTF. All of these cytokines are predicted to have a four helix bundle structure similar to that found for growth hormone, suggesting that they all evolved from a common ancestral gene (11-13).

The effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion (6, 14 - 17). IL-6 also affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression.

IL-6 exhibits growth factor activity for mature thymic or peripheral T-cells and reportedly enhances the differentiation of cytotoxic T-cells in the presence of IL-2 or IFN-γ (18-20). IL-6 stimulates production of acute phase proteins by hepatocytes (21) and has colony-stimulating activity on hematopoietic stem cells. IL-6 has growth factor activities and will stimulate the growth of myeloma/hybridoma/ plasmacytoma cells, EBV-transformed B cells, keratinocytes and mesangial cells (4, 5).

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Hirano, T. et al. (1990) in Peptide Growth Factors and their Receptors I, Sporn, M.B. and A.B.Roberts eds., Springer-Verlag, New York, p. 663.

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May, L.T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:8957.

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Yasukawa, Y. et al. (1987) EMBO J. 6:2939.

Rose, T.M. and A.G. Bruce (1991) Proc. Natl. Acad. Sci. USA 88:8641d.

Bazan, J.F. (1991) Neuron 7:197.

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Bauman, H. et al. (1984) J. Biol. Chem. 259:7331.

• Representative standard curve for IL-6 ELISA. IL-6 was diluted in serial two-fold steps in Sample Diluent.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting