• Product NameMouse TNF-α ELISA kit
• Brief DescriptionELISA Kit
• ApplicationsELISA
• Species ReactivityMs
• SpecificityNatural and recombinant Mouse TNF-α Ligand
• Crossing ReactivityNo significant interference observed with available related molecules.
• Target NameMouse TNF-α

• Detect Range: 0.02 - 1.0 ng/mL
  Sensitivity: 7pg/mL
  Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
  Sample Volume: 20 uL
  Assay Time: 3 hour
  Detection method: Colorimetric

• • Aluminium pouches with a Microwell Plate coated with antibody to mouse TNF-α (8X12)
  • 2 vials mouse TNF-α Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse TNF-α antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert

The prototype ligand of theTNFsuperfamily, TNF-α/TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis (1-4).TNF-α is produced by activated macrophages and other cell types including T and B cells, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells and some tumor cells (5-8).

Mouse TNF-α cDNA encodes a 235 amino acid (aa) residue type II membrane protein (9). The 156 aa residue soluble TNF-α is released from the C-terminus of the membrane-anchored TNF-α by proteolytic cleavage by a matrix metalloprotease (10).

The membrane-anchored form of TNF-α has been shown to have lytic activity and may also have an important role in intercellular communication (11). The biologically active TNF-α has been shown to exist as a trimer (12, 13).TNF-α is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms (14). Deficiencies in either TNF-α or its receptors can increase susceptibility to infection by intracellular pathogens (15-16). TNF- may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers (17, 18). Other potential physiological roles for TNF-α and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells (19-21).

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• Representative standard curve for TNF-α ELISA. TNF-α was diluted in serial two-fold steps in Sample Diluent.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting