• Product NameMouse IL-6 ELISA kit
• Brief DescriptionELISA Kit
• ApplicationsELISA
• Species ReactivityMs
• SpecificityNatural and recombinant Mouse IL-6 Ligand
• Crossing ReactivityNo significant interference observed with available related molecules.
• Target NameMouse IL-6

• Detect Range: 0.02 - 1.0 ng/mL
  Sensitivity: 7pg/mL
  Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
  Sample Volume: 20 uL
  Assay Time: 3 hour
  Detection method: Colorimetric

• • Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-6 (8x12)
  • 2 vials mouse IL-6 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-6 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert

Interleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis (1-5). It is expressed by a variety of normal and transformed lymphoid and nonlymphoid cells.

IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction (4). The mouse (6), rat (7) and human (8, 9) IL-6 cDNAs have been cloned. The mouse IL-6 cDNA encodes a 211 amino acid (aa) residue precursor polypeptide with a hydrophobic signal sequence that is cleaved to generate the 187 aa residue mature protein. Mouse to rat and human, there is approximately 87% and 39% aa identity, respectively. Although human and mouse IL-6 are equally active on mouse cells, mouse IL-6 is not active on human cells (10).

The high-affinity IL-6 receptor complex, which mediates IL-6 bioactivity, consists of two membrane glycoproteins. The production of IL-6 is upregulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations and malignancies (3, 5).

Hibi, M. et al. (1996) J Mol. Med. 74:1.

Hirano, T. et al. (1994) Stem Cells 12:262.

Van Snick, J. et al. (1990) Annu. Rev. Immunol. 8:253.

Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

Hirano, T. (1994) "Interleukin 6" in The Cytokine Handbook 2nd ed. Academic Press, New York, p. 145.

Chiu, C-P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.

Northemann, W. et al. (1989) J. Biol. Chem. 264:16072.

Hirano, T. et al. (1986) Nature 324:73.

Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193.

Cayphas, S. et al. (1987) J. Immunol. 139:2965.

• Representative standard curve for IL-6 ELISA. IL-6 was diluted in serial two-fold steps in Sample Diluent.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting