• Product NameMouse IL-23 ELISA kit
• Brief DescriptionELISA Kit
• ApplicationsELISA
• Species ReactivityMs
• SpecificityNatural and recombinant Mouse IL-23 Ligand
• Crossing ReactivityNo significant interference observed with available related molecules.
• Target NameMouse IL-23

• Detect Range: 0.02 - 1.0 ng/mL
  Sensitivity: 7pg/mL
  Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
  Sample Volume: 20 uL
  Assay Time: 3 hour
  Detection method: Colorimetric

• • Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-23 (8X12)
  • 2 vials mouse IL-23 Standard lyophilized, 500 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-23 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert

Interleukin 23 (IL-23) is a heterodimeric cytokine that is related to IL-12 (1-3). It is composed of two disulfide-linked subunits, a 19 kDa (p19) subunit that is unique to IL-23, and a 40 kDa (p40, IL-12) subunit that is shared with IL-12 (3-7). Mature mouse p19 and p40 share 88% and 92% aa sequence identity, respectively, with the corresponding rat subunits. IL-23 is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens including certain bacteria and viruses and/or their components (3, 6). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor-1 subunit (IL-12 Rβ1) and the IL-23-specific receptor subunit (IL-23 R) (7). IL-23 and IL-12 have overlapping and distinct biological activities. The IL-23 immune pathway induces the earliest recruitment of neutrophils to the site of infection, while the more classic host defense and cytotoxic response is stimulated by IL-12 (4). IL-23 has a role in the development and maintenance of a T cell subset, designated Th17, that is characterized by the production of IL-17A, IL-17F, IL-6, and TNF-α(3, 4, 8). The IL-23/IL-17 axis is an important mediator of inflammation. In mouse models, transgenic over-expression of IL-23 leads to a lethal systemic infl ammatory response (9). IL-23 eff ects on Th17 cells may also enhance the development of several models of autoimmune disease including experimental allergic encephalomyelitis (EAE), collagen-induced arthritis (CIA), colitis, and diabetes (5, 8, 10-14). IL-23 may also play a role in increased tumor growth associated with chronic inflammation (15).

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• Representative standard curve for IL-23 ELISA. IL-23 was diluted in serial two-fold steps in Sample Diluent.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting