• Product NameSmac Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationSmac Antibody is DEAE purified.
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to amino acids 225 to 239 of human Smac/DIABLO.
• Target NameSmac
• Alternative Names    DIABLO
• Accession No. Swiss-Prot:Q9JIQ3
  Gene ID:66593
• UniprotQ9JIQ3
• Gene ID66593;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 25 kd
  

The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.

• Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide (2409P) with Smac antibody at 1 ug /ml.
• Immunohistochemistry of Smac/DIABLO in human ovary tissue with Smac antibody at 5 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting