• Product NameLGR5 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF,IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human LGR5 (NP_003658.1).
• Alternative Names    LGR5;FEX;GPR49;GPR67;GRP49;HG38
• Accession No. Swiss Prot:O75473
  GeneID:8549
• Calculated MW 92kDa/97kDa/99kDa
• SDS-PAGE MW 105kDa
• Concentration 2.92 mg/mL
• Formulation PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:1000 - 1:2000
  IF 1:50 - 1:200

  IHC 1:50 - 1:200

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of lysates from Mouse brain using LGR5/GPR49 Rabbit pAb at 1:2000 dilution.
• Immunohistochemistry analysis of paraffin-embedded Rat brain using LGR5/GPR49 Rabbit pAb at dilution of 1:300 (40x lens).
• Immunohistochemistry analysis of paraffin-embedded Rat lung using LGR5/GPR49 Rabbit pAb at dilution of 1:300 (40x lens).
• Immunohistochemistry analysis of paraffin-embedded Rat ovary using LGR5/GPR49 Rabbit pAb at dilution of 1:300 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting