• Product NameYAP1 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human YAP1 (NP_001123617.1).
• Alternative Names    COB1;YAP;YAP2;YAP65;YKI;YAP1
• Accession No. Swiss Prot:P46937
  GeneID:10413
• UniprotP46937
• Calculated MW 36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
• SDS-PAGE MW 75kDa
• Concentration 0.8mg/ml
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

• Immunohistochemistry of paraffin-embedded rat liver using YAP1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat spleen using YAP1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human lung cancer using YAP1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human liver cancer using YAP1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human gastric cancer using YAP1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human uterus using YAP1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse heart using YAP1 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of HeLa cells using YAP1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH/3T3 cells using YAP1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of PC-12 cells using YAP1 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting