• Product NameLivin Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityA lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin.
• Immunogen TypePeptide
• Immunogen DescriptionLivin antibody was raised with a synthetic peptide corresponding to amino acids 264 to 280 of. The short form and 281 to 298 of the long form of human Livin. This sequence is identical between a and b forms of the Livin proteins.
• Target NameLivin
• Alternative Names    KIAP
• Accession No. Swiss-Prot:Q96CA5
  Gene ID:79444
• UniprotQ96CA5
• Gene ID79444;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 33 kd
  

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP. Livin/XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-alpha and Livin-beta. Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6. Livin has direct interaction with several caspases including caspase-3, -7, and -9. Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions.

• Western blot analysis of Livin expression in human Raji cell lysate with Livin antibody at 0.5 ug/mL.
• Immunohistochemistry of Livin in human small intestine tissue with Livin antibody at 5 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting