• Product NamePUMA Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IHC
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a synthetic peptide corresponding to 14 amino acids near the amino terminus of human PUMA-a. This sequence is identical between a and b forms of the PUMA proteins.
• Target NamePUMA
• Alternative Names    bbc3
• Accession No. Swiss-Prot:Q96PG8
  Gene ID:27113
• UniprotQ96PG8
• Gene ID27113;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Predicted MW: 23 kd

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

• Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at 2 ug /ml.
• Immunohistochemistry of PUMA in human breast carcinoma with PUMA antibody at 10 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting