• Product NamePyk2(Phospho-Tyr402) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Pyk2 only when phosphorylated at tyrosine 402.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 402 (D-I-Y(p)-A-E) derived from Human Pyk2.
• Target NamePyk2
• ModificationPhospho
• Alternative Names    FADK 2; FAK2; Focal adhesion kinase 2; PTK2B; Proline-rich tyrosine kinase 2 RAFTK
• Accession No. Swiss-Prot: Q14289
  NCBI Protein: NP_004094.3
• UniprotQ14289
• Gene ID2185;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 116kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.

Gluck SL, et al. (2004) J Clin Invest; 114(12): 1696-1699
Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9784-9789
Tian D, et al. (2002) Mol Cell Biol; 22(8): 2650-2662
Lu Z, et al. (2001) Mol Cell Biol; 21(12): 4016-4031
Krishnan HH, et al. (2006) J Virol; 80(3): 1167-1180

• Western blot analysis of extracts from 293 cells untreated or treated with Serum using Pyk2(Phospho-Tyr402) Antibody #11216.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Pyk2(Phospho-Tyr402) Antibody #11216(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using Pyk2(Phospho-Tyr402) Antibody #11216.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting