• Product NameG3BP2 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationThe antibody was purified by immunogen affinity chromatography.
• ApplicationsWB,IF/IC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human G3BP2. The exact sequence is proprietary.
• Alternative Names    G3BP2
• Accession No. Swiss Prot:Q9UN86
  GeneID:9908
• UniprotQ9UN86
• Calculated MW 50kDa/54kDa
• Formulation Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:1000;,br> IF/ICC 1:50 - 1:200

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• Western blot analysis of G3BP2 expression in MCF7 (A), SKOVCAR3 (B), A549 (C), C6 (D), CT26 (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54; 51 kD)
• Immunofluorescent analysis of G3BP2 staining in VB2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting