• Product Nameb-Catenin(Phospho-Ser37) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of b-Catenin only when phosphorylated at serine 37.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 37 (I-H-S(p)-G-A) derived from Human b-Catenin.
• Target Nameb-Catenin
• ModificationPhospho
• Alternative Names    CTNNB1; CATNB; CTNB1; CTNNB;
• Accession No. Swiss-Prot: P35222
  NCBI Protein: NP_001091679.1
• UniprotP35222
• Gene ID1499;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 92kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Novak A, et al. (1998) Proc Natl Acad Sci U S A; 95(8): 4374-4379
Marin O, et al. (2003) Proc Natl Acad Sci U S A; 100(18): 10193-10200
Okamura H, et al. (2004) Mol Cell Biol; 24(10): 4184-4195
Xing Y, et al. (2003) Genes Dev; 17(22): 2753-2764
Barth AI, et al. (1999) Proc Natl Acad Sci U S A; 96(9): 4947-4952

• Western blot analysis of extracts from HT29 cells using b-Catenin(Phospho-Ser37) Antibody #11219 and the same antibody preincubated with blocking peptide .
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Phospho-Ser37) Antibody #11219(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting