• Product NameBCAT2 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human BCAT2 (NP_001181.2).
• Alternative Names    BCAT2;BCAM;BCATM;BCT2;PP18
• Accession No. Swiss Prot:O15382
  GeneID:587
• UniprotO15382
• Calculated MW 33kDa/44kDa
• SDS-PAGE MW 40kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.

• Immunofluorescence analysis of NIH-3T3 cells using BCAT2 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using BCAT2 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting