• Product NameGoat Anti-Rabbit IgG,Polymer-HRP
• Brief DescriptionSecondary Antibody
• ClonalityPolyclonal
• ApplicationsELISA
• Species ReactivityRabbit
• Immunogen DescriptionRabbit IgG, heavy and light chains
• ConjugatesPolymer-horseradish peroxidase (HRP)
• Storage Supplied in 100x concentrated solution. Store at 4C.

• ELISA: 1:100-1:300

This secondary antibody is affinity-purified and is suitable to detect rabbit antibodies used in ELISA. Polymer-HRP conjugation enhances the sensitivity and reduces the background of the detection. First, a primary antibody of rabbit origin is bound to a target antigen. After a brief wash, Polymer-HRP-conjugated goat anti-rabbit IgG secondary antibody is applied to form a complex with the primary antibody. After another brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to generate detectable chemiluminescence or insoluble colors.

• Figure 1. SARS-CoV-2 chemiluminescent ELISA. Different concentrations of gamma-irradiated SARS-CoV-2 viruses were lysed in Viral Lysis Buffer (Cat# VL101) and coated (150 μL) on microplate wells. Rabbit monoclonal anti-N primary antibody (Cat# VYN7, 1:10,000 dilution) and Polymer-HRP-conjugated goat anti-rabbit IgG secondary antibody (Cat# 303, 1:100) were used to detect SARS-CoV-2 N protein. PiQTM ECL (Cat# 636) reagents were used to generate chemiluminescent signals. RLU, relative light unit.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting