• Product NameUltrasensitive Streptavidin-polymer-HRP
• Brief DescriptionSecondary Antibody
• ApplicationsELISA
• Species ReactivityBiotinylated proteins, DNA, RNA, and other macromolecules
• ConjugatesHorseradish peroxidase (HRP) and streptavidin are conjugated to hydrophilic backbones.
• Storage Supplied in PBS, pH 7.2 and 50% glycerol. This product is stable for 2 years when stored at -20˚C.

• ELISA: 1:10,000

Biotinylated proteins including primary and secondary antibodies are widely used in protein purification and immunoassays. Due to its high binding affinity to streptavidin, biotinylated proteins are often used with streptavidin conjugates such as streptavidin-fluorescent dyes and streptavidin-HRP to amply the signals and increase detection sensitivity. Streptavidin-polymer-HRP combine the specificity of streptavidin-biotin interaction and signal amplification through multiple conjugations of HRP to a hydrophilic polymer backbone. The applications of this highly sensitive detection of biotinylated proteins include Western blotting, ELISA, immunoprecipitation, immunocytochemistry and immunohistochemistry.

• Figure 1. SARS-CoV-2 chemiluminescent ELISA. Different concentrations of gamma-irradiated SARS-CoV-2 viruses were lysed in Viral Lysis Buffer (Cat# VL101) and coated (150 μL) on microplate wells. Biotin-conjugated rabbit monoclonal anti-N primary antibody (Cat# VYN7B, 1:10,000 dilution) and ultrasensitive streptavidin-polymer-HRP (Cat# 505, 1:10,000) were used to detect SARS-CoV-2 N protein. PiQTM ECL (Cat# 636) reagents were used to generate chemiluminescent signals. RLU, relative light unit.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting