Detection of SARS-COV-2 is challenging when viral abundance is low. In the first place, no test, whether it is RT-PCR or immunoassay, is 100% accurate; therefore, false negative or positive results are inevitable (Shuren and Stenzel, 2020). False negative results will increase the risk of viral spread, whereas false positive results will increase the burdens of healthcare providers and unnecessary quarantines (Shuren and Stenzel, 2020). Second, the virial load in different types of clinical specimens can vary dramatically. Clinical studies reveal that lower respiratory tract specimens have more viral load (Hu et al., 2020; Wang et al., 2020). Specifically, bronchoalveolar lavage fluid specimens contain the highest vial load, followed by sputum, nasal swabs, fibrobronchoscope brush biopsy, pharyngeal swabs, feces, and blood (Wang et al., 2020). Finally, the presence of SARS-COV-2 in water and wastewater transmitted through patients stools and masks has raised considerable environmental concerns (Tran et al., 2020). However, detecting SARS-CoV-2 at extremely low level is technically difficult. To this end, we have developed GenuIN? CoV2 Enrichment Kit to concentrate SARS-CoV-2 nucleocapsid proteins in solutions using magnetic beads. The enriched N proteins will be used for subsequent applications such as immunoprecipitation, ELISA, lateral flow immunoassay, and proteomics research.
