• Product Name96-Well Microplate
• Brief DescriptionReagent
• ApplicationsELISA
• Storage Room temperature; dry

• RECOMMENDED WORKING VOLUME: 100-230 mL

MxBind 96-well polystyrene microplate is pre-treated to have high-binding capacity for proteins and biomolecules with both hydrophilic and hydrophobic structures. It is also optimized to bind high amounts of immunoglobins, suitable for sandwich ELISA assays. MxBind? 96-well polystyrene microplate has been tested in chemiluminescent ELISA and used in SARS-CoV-2 antigen detection and enrichment kits.

• Comparison of ShQ™ ECL and PiQ™ ECL sensitivity using MxBind™ 96-well polystyrene microplate (Cat# VE101) wells. Different concentrations of gamma-irradiated SARS-CoV-2 viruses were lysed in Viral Lysis Buffer (Cat# VL101) and coated (150 μL) on MxBind™ 96-well polystyrene microplate (Cat# VE101) wells. Rabbit monoclonal anti-N primary antibody (Cat# VYN7, 1:10,000 dilution) and HRP-conjugated highly cross-adsorbed goat anti rabbit IgG secondary antibody (Cat# 202, 1:20,000) were used to detect SARS-CoV-2 N protein. ShQTM ECL (Cat# 707) and PiQTM ECL (Cat# 636) Substrate Kits were used to generate chemiluminescent signals. RLU, relative light unit. ΔRLU = RLU of the well with primary antibody - RLU of the corresponding well without primary antibody, all else being equal. Note: when antigen is too abundant, ShQTM ECL is a better choice based on the linear regression data.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting