• Product NameECL SUBSTRATE KIT
• Brief DescriptionReagent
• ApplicationsChemiluminescent ELISA
• Storage Stable at 4˚C for at least 1 year.

• RECOMMENDED ANTIBODY DILUTIONS: Primary antibody: 1: 5,000 - 1:10,000; Secondary antibody: 1: 10,000 - 1:20,000

Enhanced chemiluminescence (ECL) reagents are used with horseradish peroxidase (HRP)-conjugated secondary antibodies to detect protein abundance in immunoassays. HRP catalyzes luminol oxidation in the presence of hydrogen peroxide, producing light that can be detected by X-ray film, digital imagers or chemiluminescence plate reader. ShQ? ECL Substrate Kit is proprietarily formulated for chemiluminescent ELISA. When compared to ultrasensitive PiQ? ECL, ShQ? ECL exhibit superior performance by generating extremely low background.

• Comparison of ShQ™ ECL and PiQ™ ECL sensitivity in chemiluminescent ELISA. Different concentrations of gamma-irradiated SARS-CoV-2 viruses were lysed in Viral Lysis Buffer (Cat# VL101) and coated (150 μL) on microplate wells. Rabbit monoclonal anti-N primary antibody (Cat# VYN7, 1:10,000 dilution) and HRP-conjugated highly cross-adsorbed goat anti rabbit IgG secondary antibody (Cat# 202, 1:20,000) were used to detect SARS-CoV-2 N protein. ShQTM ECL (Cat# 707) and PiQTM ECL (Cat# 636) Substrate Kits were used to generate chemiluminescent signals. RLU, relative light unit. ΔRLU = RLU of the well with primary antibody - RLU of the corresponding well without primary antibody, all else being equal. Note: when antigen is too abundant, ShQTM ECL is a better choice based on the linear regression data.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting