• Product NameWee 1 (phospho Ser53) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-Wee 1 (S53) Polyclonal Antibody detects endogenous levels of Wee 1 protein only when phosphorylated at S53.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser53. AA range:19-68
• Alternative Names    WEE1; Wee1-like protein kinase; WEE1hu; Wee1A kinase
• Accession No. Swiss Prot:P30291
  GeneID:7465
• UniprotP30291
• Gene ID7465
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• 
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/20000. Not yet tested in other applications.

WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting