• Product NameThymidine Kinase (phospho Ser13) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman,Mouse,Rat,Pig(Test by out customer)
• SpecificityPhospho-Thymidine Kinase (S13) Polyclonal Antibody detects endogenous levels of Thymidine Kinase protein only when phosphorylated at S13.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human TK around the phosphorylation site of Ser13. AA range:1-50
• Alternative Names    TK1; Thymidine kinase; cytosolic
• Accession No. Swiss Prot:P04183
  GeneID:7083
• UniprotP04183
• Gene ID7083
• SDS-PAGE MW 25
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/5000. Not yet tested in other applications.

catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family.,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting