• Product NameRPA32 (phospho Thr21) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman,Mouse
• SpecificityPhospho-RPA32 (T21) Polyclonal Antibody detects endogenous levels of RPA32 protein only when phosphorylated at T21.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Thr21. AA range:10-59
• Alternative Names    RPA2; REPA2; RPA32; RPA34; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34
• Accession No. Swiss Prot:P15927
  GeneID:6118
• UniprotP15927
• Gene ID6118
• SDS-PAGE MW 35
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/10000. Not yet tested in other applications.

function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting