• Product NamePRK1 (phospho-Thr774)/PRK2 (phospho-Thr816) rabbit pAb
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• SpecificityThis antibody detects endogenous levels of Human Mouse Rat PRK1 (phospho-Thr774) or PRK2 (phospho-Thr816)
• Immunogen DescriptionSynthesized phosho peptide around human PRK1 (Thr774) and PRK2 (Thr816)
• Alternative Names    Serine/threonine-protein kinase N1 (EC 2.7.11.13) (Protease-activated kinase 1) (PAK-1) (Protein kinase C-like 1) (Protein kinase C-like PKN) (Protein kinase PKN-alpha) (Protein-kinase C-related kinase 1) (Serine-threonine protein kinase N)
• Accession No. Swiss Prot:Q16512
  GeneID:5585
• UniprotQ16512
• Gene ID5585
• SDS-PAGE MW 103
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• WB 1:1000-2000

protein kinase N1(PKN1) Homo sapiens The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting