• Product Namep27 (phospho Thr157) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman
• SpecificityPhospho-p27 (T157) Polyclonal Antibody detects endogenous levels of p27 protein only when phosphorylated at T157.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Thr157. AA range:123-172
• Alternative Names    CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
• Accession No. Swiss Prot:P46527
  GeneID:1027
• UniprotP46527
• Gene ID1027
• Calculated MW 22kd
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• 
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/5000. Not yet tested in other applications.

cyclin dependent kinase inhibitor 1B(CDKN1B) Homo sapiens This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting