• Product NameMyf-5 (phospho Ser49) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman,Mouse
• SpecificityPhospho-Myf-5 (S49) Polyclonal Antibody detects endogenous levels of Myf-5 protein only when phosphorylated at S49.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human Myogenic Factor 5 around the phosphorylation site of Ser49. AA range:21-70
• Alternative Names    MYF5; BHLHC2; Myogenic factor 5; Myf-5; Class C basic helix-loop-helix protein 2; bHLHc2
• Accession No. Swiss Prot:P13349
  GeneID:4617
• UniprotP13349
• Gene ID4617
• SDS-PAGE MW 38
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/10000. Not yet tested in other applications.

function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting