• Product NameMAD1 (phospho Ser428) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman
• SpecificityPhospho-MAD1 (S428) Polyclonal Antibody detects endogenous levels of MAD1 protein only when phosphorylated at S428.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human MAD1 around the phosphorylation site of Ser428. AA range:394-443
• Alternative Names    MAD1L1; MAD1; TXBP181; Mitotic spindle assembly checkpoint protein MAD1; Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181
• Accession No. Swiss Prot:Q9Y6D9
  GeneID:8379
• UniprotQ9Y6D9
• Gene ID8379
• Calculated MW 83kd
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• 
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/5000. Not yet tested in other applications.

MAD1 mitotic arrest deficient like 1(MAD1L1) Homo sapiens MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting