• Product NameERF (phospho Thr526) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF/ICC,ELISA
• Species ReactivityHuman,Mouse
• SpecificityPhospho-ERF (T526) Polyclonal Antibody detects endogenous levels of ERF protein only when phosphorylated at T526.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human ERF around the phosphorylation site of Thr526. AA range:492-541
• Alternative Names    ERF; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE-2
• Accession No. Swiss Prot:P50548
  GeneID:2077
• UniprotP50548
• Gene ID2077
• SDS-PAGE MW 58
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: 1/20000. Not yet tested in other applications.

ETS2 repressor factor(ERF) Homo sapiens ETS2 is a transcription factor and protooncogene involved in development, apoptosis, and the regulation of telomerase. The protein encoded by this gene binds to the ETS2 promoter and is a strong repressor of ETS2 transcription. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Aug 2015],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting