• Product Namec-Fos (phospho Ser374) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC-p,IF(paraffin section),WB,ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-c-Fos (S374) Polyclonal Antibody detects endogenous levels of c-Fos protein only when phosphorylated at S374.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human Fos around the phosphorylation site of Ser374. AA range:331-380
• Alternative Names    FOS; G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7
• Accession No. Swiss Prot:P01100
  GeneID:2353
• UniprotP01100
• Gene ID2353
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• WB 1:500-2000
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/10000. Not yet tested in other applications.

Fos proto-oncogene, AP-1 transcription factor subunit(FOS) Homo sapiens The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting