• Product NameC3G (phospho Tyr504) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman
• SpecificityPhospho-C3G (Y504) Polyclonal Antibody detects endogenous levels of C3G protein only when phosphorylated at Y504.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human RapGEF1 around the phosphorylation site of Tyr504. AA range:470-519
• Alternative Names    RAPGEF1; GRF2; Rap guanine nucleotide exchange factor 1; CRK SH3-binding GNRP; Guanine nucleotide-releasing factor 2; Protein C3G
• Accession No. Swiss Prot:Q13905
  GeneID:2889
• UniprotQ13905
• Gene ID2889
• SDS-PAGE MW 120
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  ELISA: 1/10000. Not yet tested in other applications.

Rap guanine nucleotide exchange factor 1(RAPGEF1) Homo sapiens This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting