• Product NameB23 (phospho Thr199) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF/ICC,ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-B23 (T199) Polyclonal Antibody detects endogenous levels of B23 protein only when phosphorylated at T199.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220
• Alternative Names    NPM1; NPM; Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin
• Accession No. Swiss Prot:P06748
  GeneID:4869
• UniprotP06748
• Gene ID4869
• SDS-PAGE MW 32
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: 1/40000. Not yet tested in other applications.

nucleophosmin(NPM1) Homo sapiens This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting