• Product NameA-Raf (phospho Tyr302) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF/ICC,ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-A-Raf (Y302) Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at Y302.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302. AA range:276-325
• Alternative Names    ARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
• Accession No. Swiss Prot:P10398
  GeneID:369
• UniprotP10398
• Gene ID369
• SDS-PAGE MW 68
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: 1/20000. Not yet tested in other applications.

A-Raf proto-oncogene, serine/threonine kinase(ARAF) Homo sapiens This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting