• Product NameAP-1 (phospho Thr231) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC-p,IF/ICC,ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-AP-1 (T231) Polyclonal Antibody detects endogenous levels of AP-1 protein only when phosphorylated at T231.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human c-Jun around the phosphorylation site of Thr231. AA range:201-250
• Alternative Names    JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39
• Accession No. Swiss Prot:P05412
  GeneID:3725
• UniprotP05412
• Gene ID3725
• SDS-PAGE MW 39-42
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• 
  Immunohistochemistry: 1/100 - 1/300.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: 1/10000. Not yet tested in other applications.

Jun proto-oncogene, AP-1 transcription factor subunit(JUN) Homo sapiens This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting