• Product NameAIP4 (phospho Tyr420) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman,Mouse
• SpecificityPhospho-AIP4 (Y420) Polyclonal Antibody detects endogenous levels of AIP4 protein only when phosphorylated at Y420.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420. AA range:386-435
• Alternative Names    ITCH; E3 ubiquitin-protein ligase Itchy homolog; Itch; Atrophin-1-interacting protein 4; AIP4; NFE2-associated polypeptide 1; NAPP1
• Accession No. Swiss Prot:Q96J02
  GeneID:83737
• UniprotQ96J02
• Gene ID83737
• Calculated MW 102kd
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• 
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/20000. Not yet tested in other applications.

itchy E3 ubiquitin protein ligase(ITCH) Homo sapiens This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting