• Product Name5-LO (phospho Ser523) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,ELISA
• Species ReactivityHuman,Rat
• SpecificityPhospho-5-LO (S523) Polyclonal Antibody detects endogenous levels of 5-LO protein only when phosphorylated at S523.
• Immunogen DescriptionSynthesized phospho-peptide around the phosphorylation site of human 5-LO (phospho Ser523)
• Alternative Names    ALOX5; LOG5; Arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase
• Accession No. Swiss Prot:P09917
  GeneID:240
• UniprotP09917
• Gene ID240
• SDS-PAGE MW 77
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  ELISA: 1/10000. Not yet tested in other applications.

arachidonate 5-lipoxygenase(ALOX5) Homo sapiens This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting