• Product NameHRP-conjugated goat anti-mouse IgG secondary antibody
• Brief DescriptionSecondary Antibody
• ClonalityPolyclonal
• ApplicationsWB ELISA
• Species ReactivityMouse IgG
• Immunogen DescriptionMouse IgG, heavy and light chains
• ConjugatesHorseradish peroxidase (HRP)
• Storage Supplied in PBS, pH 7.2 and 50% glycerol. This product is stable for 2 years when stored at - 20°C.

• Western blotting (WB): 1:2,000

  ELISA: 1:20,000

HRP-conjugated goat anti-mouse IgG secondary antibody is affinity-purified and is suitable for detecting mouse primary antibodies used in WB, IHC and ELISA. First, a primary antibody of mouse origin is bound to a target antigen. Following a brief wash, HRP-conjugated goat antimouse secondary antibody is applied to form a complex with the primary antibody. After another brief wash, HRP substrates such as luminol or 3,3'-diaminobenzidine (DAB) are added to produce detectable chemiluminescence or insoluble colors. 

• HEK 293 cell lysates were loaded as indicated and separated by SDS-PAGE. Anti-ACTB(β-actin) mouse monoclonal primary(1:5000) and HRP-conjugated goat anti-mouse secondary antibody(1:20,000) were applied. To detect the signal,the membrane was incubated with PiQ™ ECL, and the image was captured with a ChemiDoc XRS+diqital imaqer.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting