• Product NameStathmin1(Phospho-Ser25) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Stathmin 1 only when phosphorylated at serine 25.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 25 (I-L-S(p)-P-R) derived from Human Stathmin 1.
• Target NameStathmin1
• ModificationPhospho
• Alternative Names    STMN1; STN1; stathmin
• Accession No. Swiss-Prot: P16949
  NCBI Protein: NP_001138926.1
• UniprotP16949
• Gene ID3925;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 19kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear

Boehm M, et al. (2002) EMBO J 21(13): 3390-3401.
Vadlamudi RK, et al. (2005) Mol Cell Biol 25(9): 3726-3736.
Zilfou JT, et al. (2001) Mol Cell Biol 21(12): 3974-3985.
Biernat J, et al. (2002) Mol Biol Cell 13(11): 4013-4028.

• Western blot analysis of extracts from Jurkat cells untreated or treated with PMA using Stathmin 1(Phospho-Ser25) Antibody #11224.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1(Phospho-Ser25) Antibody #11224(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting