• Product NameEIF4A1 (Succinyl-K309) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• ApplicationsWB
• Species ReactivityHuman
• SpecificityEIF4A1(Succinyl-K309) Antibody detects endogenous levels of eIF4A1 only when succinylated at K309.
• Immunogen DescriptionA synthesized peptide derived from human eIF4A1 around the succinylated site of K309.
• Alternative Names    eIF-4A-I,eIF4A-I,DDX2A, EIF4A
• UniprotP60842
• Gene ID1973
• SDS-PAGE MW 46kDa
• Concentration 1 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.
• Storage Store at --20˚C/1 year

• Western Blot: 1/500 - 1/2000

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting