• Product NameGLUT1(Mono-methyl-K245) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• ApplicationsWB
• Species ReactivityHuman
• SpecificityGLUT1(Mono-methyl-K245) Antibody detects endogenous levels of GLUT1 only when mono-methylated at K245.
• Immunogen DescriptionA synthesized peptide derived from human GLUT1 around the mono-methylated site of K245.
• Alternative Names    Glucose transporter type 1, erythrocyte/brain,GLUT-1,HepG2 glucose transporter,GLUT1
• UniprotP11166
• Gene ID6513
• SDS-PAGE MW 54kDa
• Concentration 1 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.
• Storage Store at --20˚C/1 year

• Western Blot: 1/500 - 1/2000

Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed:18245775, PubMed:19449892, PubMed:25982116, PubMed:27078104, PubMed:10227690). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed:18245775, PubMed:19449892). Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed:10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting