• Product NameCHKA (Acetyl-Lys452) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• ApplicationsWB
• Species ReactivityHM
• SpecificityCHKA(Acetyl-Lys452) Antibody detects endogenous levels of CHKA only when acetylated at Lys452.
• Immunogen DescriptionA synthesized peptide derived from human CHKA around the acetylation site of Lys452.
• Alternative Names    Choline kinase alpha; CK; CHETK-alpha; Ethanolamine kinase; EK; CHK; CKI
• UniprotP35790
• Gene ID1119
• SDS-PAGE MW 52kDa
• Concentration 1 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.
• Storage Store at --20˚C/1 year

• Western Blot: 1/500 - 1/2000

Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting