• Product NamePGK1 (Acetyl-K279) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• ApplicationsWB
• Species ReactivityHuman,Mouse
• SpecificityPGK1 (Acetyl-K279) Antibody detects endogenous levels of PGK1 only when acetylated at lysine 279.
• Immunogen DescriptionA synthesized peptide derived from human PGK1 around the phosphorylation site of K279.
• Alternative Names    Phosphoglycerate kinase 1,Cell migration-inducing gene 10 protein,Primer recognition protein 2, PRP 2,PGKA
• UniprotP00558
• Gene ID5230
• SDS-PAGE MW 45kDa
• Concentration 1 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.
• Storage Store at --20˚C/1 year

• Western Blot: 1/500 - 1/2000

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting