• Product NameSQSTM1/p62 (Phospho-Ser) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• ApplicationsWB
• Species ReactivityHuman
• SpecificitySQSTM1/p62(Phospho-Ser28) Antibody detects endogenous levels of NFKBIA only when phosphorylated at Ser28.
• Immunogen DescriptionA synthesized peptide derived from human SQSTM1/p62 around the phosphorylation site of Ser28.
• Alternative Names    SQSTM1,ORCA, OSIL,Sequestosome-1,Ubiquitin-binding protein p62,EBIAP
• UniprotQ13501
• Gene ID8878
• Calculated MW 47kd
• Concentration 1 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.
• Storage Store at --20˚C/1 year

• Western Blot: 1/500 - 1/2000

Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family (PubMed:16286508, PubMed:20168092, PubMed:24128730, PubMed:28404643, PubMed:22622177). Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting