• Product NameCKB (Phospho-Ser199) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• ApplicationsWB
• Species ReactivityH
• SpecificityCKB(Phospho-Ser199) Antibody detects endogenous levels of CKB only when phosphorylated at Ser199.
• Immunogen DescriptionA synthesized peptide derived from human CKB around the phosphorylation site of Ser199.
• Alternative Names    Creatine kinase B-type; Brain creatine kinase; B-CK; Creatine kinase B chain; Creatine phosphokinase B-type;CPK-B; CKBB
• UniprotP12277
• Gene ID1152
• SDS-PAGE MW 43kDa
• Concentration 1 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.
• Storage Store at --20˚C/1 year

• Western Blot: 1/500 - 1/2000

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting