• Product NameNEK7 (Phospho-Ser204) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• ApplicationsWB
• Species ReactivityMouse
• SpecificityNEK7 (Phospho-Ser204) Antibody detects endogenous levels of NEK7 only when phosphorylated at serine 204.
• Immunogen DescriptionA synthesized peptide derived from human NEK7 around the phosphorylation site of S204.
• Alternative Names    Serine/threonine-protein kinase Nek7,Never in mitosis A-related kinase 7,NimA-related protein kinase 7
• UniprotQ8TDX7
• Gene ID140609
• SDS-PAGE MW 35kDa
• Concentration 1 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide.
• Storage Store at --20˚C/1 year

• Western Blot: 1/500 - 1/2000

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596). Phosphorylates RPS6KB1 (By similarity). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting