• Product NameNFKBIA (Phospho-Thr291) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• ApplicationsWB
• Species ReactivityHuman
• SpecificityNFKBIA (Phospho-Thr291) Antibody detects endogenous levels of NFKBIA only when phosphorylated at threonine 291.
• Immunogen DescriptionA synthesized peptide derived from human NFKBIA around the phosphorylation site of T291.
• Alternative Names    NF-kappa-B inhibitor alpha,I-kappa-B-alpha,IkB-alpha, IkappaBalpha
• UniprotP25963
• Gene ID4792
• SDS-PAGE MW 36kSa
• Concentration 1 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide.
• Storage Store at --20˚C/1 year

• Western Blot: 1/500 - 1/2000

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting