• Product NameRecombinant human IDO1
• Host SpeciesE.coli
• Purification> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
• Biological ActivityMeasured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is >500 pmol/min/μg, as measured under the described conditions.
• Endotoxin< 1 EU/μg of the protein by LAL method.
• Immunogen DescriptionMet1-Gly403
• Target NameIDO1
• Alternative Names    3dioxygenase; EC 1.13.11.52; IDO; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; Indoleamine 2; indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase
• Accession No. Uniprot:P14902
  Gene ID:3620
• UniprotP14902
• Gene ID3620
• Target Species human
• Calculated MW 45 KDa
• Tag Info N-6*His-Thrombin
• Formulation 0.22 μm filtered solution of PBS, pH 7.4.
• Reconstitution 0.2 mg/mL in sterile PBS, pH7.4
• Storage Aliquot and store at -80˚C. Avoid repeated freeze/thaw cycles.

Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N?formyl?kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN? gamma, IFN? alpha, IFN? beta, and IL?10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3?5).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting