• Product NameRecombinant human Trop2
• Host SpeciesHEK293
• Purification> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
• Biological ActivityMeasured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells or LS180 human colorectal adenocarcinoma cells. When 1 x 104 to 5 x 104 cells/well are added to Recombinant Human TROP?2 Fc Chimera coated plates (10 ��g/mL with 100 ��L/well), >45% cells will adhere after 1 hour incubation.
• Endotoxin< 1 EU/μg of the protein by LAL method.
• Immunogen DescriptionHis27-Thr274
• Target NameTrop2
• Alternative Names    Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2
• Accession No. Uniprot:P09758
  Gene ID:4070
• UniprotP09758
• Gene ID4070
• Target Species human
• Calculated MW 28.7 KDa
• Tag Info C-His-Tag
• Formulation 0.22 μm filtered solution of PBS, pH 7.4.
• Reconstitution 0.2 mg/mL in sterile PBS, pH7.4
• Storage Aliquot and store at -80˚C. Avoid repeated freeze/thaw cycles.

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting