• Product NameCaspase-5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB
• Species ReactivityHu
• SpecificityDepending on cell lines or tissues used, other cleavage products may be observed.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 16 amino acid peptide from the amino-terminus of human Caspase-5.
• Target NameCaspase-5
• Alternative Names    ICH-3, ICE _{rel<, sub> III}
• Accession No. P51878
• UniprotP51878
• Gene ID838;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.

• Western blot analysis of Caspase-5 in (A) human spleen tissue and (B) human thymus lysate with Caspase-5 antibody at 1 μg/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting