• Product NameRecombinant human Fizzy-related protein homolog
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• PurificationGreater than 90% as determined by SDS-PAGE.
• Immunogen DescriptionExpression Region:1-493aa
  Sequence Info:Full Length of Isoform 2
• Alternative Names    CDC20-like protein 1Cdh1,Hct1 homolog ;hCDH1
• Accession No. Q9UM11
• UniprotQ9UM11
• Gene ID51343;
• Calculated MW 70.8 kDa
• Tag Info N-terminal 6xHis-SUMO-tagged
• Target Sequence MDQDYERRLLRQIVIQNENTMPRVTEMRRTLTPASSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKSPSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIEKVQDPQTEDRRLQPSTPEKKGLFTYSLSTKRSSPDDGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVYLWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWNAEQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQYTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKESVSVLNLFTRIR
• Formulation Tris-based buffer50% glycerol
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
  Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Key regulator of ligase activity of the anaphase promoting complex,cyclosome (APC,C), which confers substrate specificity upon the complex. Associates with the APC,C in late mitosis, in replacent of CDC20, and activates the APC,C during anaphase and telophase. The APC,C rains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate praturely. At the G1,S transition FZR1 is phosphorylated, leading to its dissociation from the APC,C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC,C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Activation of the human anaphase-promoting complex by proteins of the CDC20,Fizzy family.Kramer E.R., Gieffers C., Hoelzl G., Hengstschlaeger M., Peters J.-M.Curr. Biol. 8:1207-1210(1998)
Research Topic:Cell Biology

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting